Functional Unit of the RNA Polymerase II C-Terminal Domain Lies within Heptapeptide Pairs

Functions of Mediator and the RNA Polymerase II C-terminal Domain in TranscriptiFunctions of Mediator and the RNA Polymerase II C-terminal Domain in Transcription Initiation

Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing.

The largest subunit of RNA polymerase II contains a unique C-terminal domain important for coupling of transcription and mRNA processing. This domain consists of a repeated heptameric sequence (YSPTSPS) phosphorylated at serines 2 and 5. Serine 5 is phosphorylated during initiation and recruits capping enzyme. Serine 2 is phosphorylated during elongation by the Ctk1 kinase, a protein similar to...

Selective constraint and the evolution of the RNA Polymerase II C-Terminal Domain

In recent years a great deal of biochemical and genetic research has focused on the C-terminal domain (CTD) of the largest subunit (RPB1) of DNA-dependent RNA polymerase II. This strongly conserved domain of tandemly repeated heptapeptides has been linked functionally to important steps in the initiation and processing of mRNA transcripts in both animals and fungi. Although they are absolutely ...

Cross-Talk of Phosphorylation and Prolyl Isomerization of the C-terminal Domain of RNA Polymerase II

Post-translational modifications of the heptad repeat sequences in the C-terminal domain (CTD) of RNA polymerase II (Pol II) are well recognized for their roles in coordinating transcription with other nuclear processes that impinge upon transcription by the Pol II machinery; and this is primarily achieved through CTD interactions with the various nuclear factors. The identification of novel mo...

Functional Analysis of the RNA Polymerase II C-terminal Domain Kinase Ctk1 in the Yeast Saccharomyces cerevisiae

Acknowledgements This work would not have been possible without the help of many, many people. Especially, I would like to thank… …Katja Sträßer for giving me the opportunity to work on this fascinating project, for every day's support, for the open door, for teaching me genetics, for the skating tours… and the sweets during the frustrating times… …Ralf-Peter Jansen for being my " Doktorvater "...